Developing a robust protein production pipeline will be instrumental in providing the key reagents needed to implement the fastest, cleanest, and most accurate DNA synthesis system in the world. As a Scientist in the Protein Production team, you will have the exciting opportunity to transition our protein production from research to pilot scale and beyond. You’ll establish the tools needed for this transition, have the exciting opportunity to take part in a transition from research to , build a team to support the work, establish tools, systems and protocols to increase production of our key reagents.

If you get goosebumps scaling up processes to generate gobs of highly pure, functional proteins, then we want to hear from you!

Responsibilities

• Design, onboard equipment for, and implement scale-up of lysis, homogenization, clarification, chromatographic purification, and final buffer exchange to produce gram-scale batches of high quality, pure protein
• Innovate on currently implemented downstream processes to drive down costs, decrease time of production, and improve purity and yield
• Use experienced judgment to guide decision making regarding pipeline development as it relates to cost-benefit analysis, operational and technical capabilities
• Help guide junior associates in downstream processing activities
• Collaborating in a team environment: At Ansa, communication between teams is critical–our technology is built on enzymes, chemistry, and automation. Additionally, we believe that kind-natured honesty and openness are an instrumental part of the scientific endeavor.

Experience

• PhD in Biochemistry, Microbiology, Bioengineering, Chemical Engineering or equivalent or Bachelor’s/Master’s with 5-10+ years of industry experience
• 2-5 years experience in downstream processing of proteins out of microbes, including gram-level scale
• Experience in recombinant protein production
• Experience with lysis using high shear techniques (eg. Microfluidizer), clarification (depth filtration or continuous flow centrifugation), column packing (eg. AxiChrom, BPG, INdEX), protein purification (AKTA FPLC, various chromatographic steps), and buffer exchange (TFF) at gram-level scale, with sub-kilogram level scale preferred
• Experience with microbial expression systems
• Experience managing 100+L scale buffer preparation a plus
• Experience with quality control assays a plus
• Experience with high density microbial fermentation a plus
• Experience with DNA-binding proteins a plus
• Enthusiasm to learn new techniques and curiosity to explore new areas of biology